Single-base mapping of m6A by an antibody-independent method
نویسندگان
چکیده
منابع مشابه
5′ UTR m6A Promotes Cap-Independent Translation
Protein translation typically begins with the recruitment of the 43S ribosomal complex to the 5' cap of mRNAs by a cap-binding complex. However, some transcripts are translated in a cap-independent manner through poorly understood mechanisms. Here, we show that mRNAs containing N(6)-methyladenosine (m(6)A) in their 5' UTR can be translated in a cap-independent manner. A single 5' UTR m(6)A dire...
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Nucleosome positioning along the DNA sequence is often viewed as a combination of two distinct aspects – translational positioning and rotational positioning. With low-resolution ( 10–20 bases) mapping techniques such as MNase digestion of chromatin, only the approximate translational position of the nucleosomes is determined. Rotational positioning can be evaluated only when the outward/inward...
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Denaturing gradient gel method for mapping single base changes in human mitochondrial DNA.
A denaturing gradient gel electrophoresis (DGGE) method is described that detects even single base pair changes in mitochondrial DNA (mtDNA). In this method, restriction fragments of mtDNA are electrophoresed in a urea/formamide gradient gel at 60 degrees C. Migration distance of each mtDNA fragment in the gel depends on melting behavior which reflects base composition. Fragments are located by...
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ژورنال
عنوان ژورنال: Science Advances
سال: 2019
ISSN: 2375-2548
DOI: 10.1126/sciadv.aax0250